The synagogue was built at the end of the 16th century as a private house of prayer for the use of mayor Mordechai Maisel and his family.

It serves today as a Jewish Museum, part of the collection of the Jewish Museum in Prague.

There is placed a collection of silver objects in the centre of the exhibition, mainly the baroque levitical set, originaly from Pinkas synagogue. There are also exposed czech dinars, from 10th and 11th century, on whose coinage the Jewish moneyers participated. There is an interesting bill of debt from the year 1378, where, as a guarantee is stated Jan Zizka from Trocnov. Very impressive are also the images showing kinds of Jews discrimination, illustrationing antisemitic stereotypes. Other parts of the exposition are dedicated to scholars Akiva Hrovic, David Oppenheim, to theirs lives and work, to historian and astronomer David Gans, or to polyhistorian Josef Selomo Delmedigo.